Context for question 1
Cells from the root of a beet plant contain a red pigment.
A student investigated the effect of surface area on the diffusion of pigment out of cylinders of beetroot tissue.
The student was provided with four identical cylinders of beetroot tissue that had been cut to 4 cm in length, and a beaker of hot water at .
The student used this method:
step 1 Label four test-tubes 1, 2, 4 and 8.
step 2 Put one of the beetroot cylinders into test-tube 1.
step 3 Put 10 of hot water into test-tube 1 and put a bung in the top of the test-tube.
step 4 Start the stop-clock. Shake the test-tube for two minutes.
step 5 After two minutes, stop the stop-clock.
step 6 Remove and discard the beetroot cylinder from test-tube 1 so that only the liquid remains in the test-tube.
step 7 Use the scalpel and ruler to cut a second beetroot cylinder into two pieces of equal length, as shown in Figure 1.1.
Put both of the pieces of beetroot into test-tube 2.
step 8 Put 10 of hot water into test-tube 2 and put a bung in the top of the test-tube.
step 9 Start the stop-clock. Shake test-tube 2 until the colour of the liquid in test-tube 2 is the same colour as the liquid in test-tube 1.
Stop the stop-clock and record the time.
step 10 Cut the third beetroot cylinder into four pieces of equal length. Put the four pieces of beetroot into test-tube 4.
step 11 Repeat steps 8 and 9 with test-tube 4.
step 12 Cut the fourth beetroot cylinder into eight pieces of equal length. Put the eight pieces of beetroot into test-tube 8.
step 13 Repeat steps 8 and 9 with test-tube 8.
step 14 Measure the final temperature of the hot water in the beaker and record this measurement.