May/June 2026 Paper 43

2026 · 6 questions · 34 parts · 80 marks

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6(a)Human Nutrition DigestionEasy2 marks
Lipase is an enzyme found in the human digestive system. Complete the word equation to show the substrate and products for a reaction catalysed by lipase.
fatty acids
6(b)(i)Enzymes Enzyme KineticsHard4 marks
There are different types of lipase. Figure 6.1 shows the effect of pH on the enzyme activity of two types of lipase, type P and type Q.
Figure 6.1, a plotted graph of enzyme activity / arbitrary units (0 to 14) against pH (3 to 12), with a key: type P lipase (solid line) is 0 at pH 3, 1 at pH 4, 8 at pH 5, peaks at 14 at pH 6, then 9 at pH 7, 2 at pH 8, 1 at pH 9 and 0 from pH 10 to 12; type Q lipase (dashed line) is 0 at pH 3 and 4, just above 0 at pH 5, 1 at pH 6, 4 at pH 7, peaks at 10 at pH 8, then 6 at pH 9, 4 at pH 10, 2 at pH 11 and 0 at pH 12
Describe two similarities and two differences between the effect of pH on the enzyme activity of type P lipase and type Q lipase. similarity 1 similarity 2 difference 1 difference 2

Answer

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6(b)(ii)Tables GraphsEasy1 mark
Using Figure 6.1, identify the pH values where type Q lipase has 50% of its maximum activity.

Two answers required.

6(b)(iii)Enzymes Enzyme KineticsMedium3 marks
Explain the effect of pH on the enzyme activity of type P lipase shown in Figure 6.1.

Answer

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6(c)(i)Human Nutrition DigestionEasy1 mark
In the small intestine, bile provides a suitable pH for type Q lipase. State one other function of bile.
6(c)(ii)Human Nutrition DigestionEasy1 mark
State the name of the organ that produces bile.