Glucose isomerase is an enzyme often used in commercial food production as it catalyses the conversion of glucose to fructose, which has a sweeter taste. The enzyme is immobilised onto a surface or within agarose beads as part of this process.

The reaction is carried out between

55^{\circ} C

and

60^{\circ} C

Which statements explain why carrying out the reaction at this temperature is beneficial?

1
Immobilised enzymes contain more active sites at higher temperatures.
2
The activation energy of the reaction is more likely to be reached.
3
Increased kinetic energy results in more collisions between enzyme and substrate.
4
Maintaining this temperature is cheaper than carrying it out at lower temperatures.