2Microscopy MagnificationMedium1 mark
A student prepared a microscope slide of onion tissue by:
- using forceps to remove a single layer of cells from the inside of a section of onion
- placing the sample on a microscope slide so it was flat and not folded on itself
- adding a few drops of iodine solution
- removing excess iodine solution using a tissue
- placing the slide on the stage of a light microscope and viewing it with a objective lens and then a objective lens.
Which problems may occur with this method?
- 1The onion tissue may dry out over time, damaging the cells.
- 2It is not possible to observe the nuclei of the onion cells.
- 3The onion tissue is too thick to allow light to penetrate through it.
- 4The onion tissue may leave a stain on the lens if the lens is lowered too close.